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  • Featured Product
  • KD/KO Validated

CAPG-Specific Polyclonal antibody

CAPG-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19535-1-AP

Synonyms

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using CAPG-Specific Polyclonal antibody (19535-1-AP)

    WB analysis of mouse lung using 19535-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using CAPG-Specific Polyclonal antibody (19535-1-AP)

    WB analysis of mouse kidney using 19535-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CAPG-Specific Polyclonal antibody (19535-1-AP)

    WB analysis of U-937 using 19535-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CAPG-Specific Polyclonal antibody (19535-1-AP)

    WB analysis of U-937 using 19535-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CAPG-Specific Polyclonal antibody (19535-1-AP)

    WB analysis of U-937 using 19535-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CAPG-Specific Polyclonal antibody (19535-1-AP)

    IHC staining of human spleen using 19535-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CAPG-Specific Polyclonal antibody (19535-1-AP)

    IHC staining of human spleen using 19535-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CAPG-Specific Polyclonal antibody (19535-1-AP)

    IHC staining of human stomach cancer using 19535-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CAPG-Specific Polyclonal antibody (19535-1-AP)

    IHC staining of human stomach cancer using 19535-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inmouse lung tissue, mouse kidney tissue, U-937 cells
Positive IHC detected inhuman spleen tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19535-1-AP targets CAPG-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name capping protein (actin filament), gelsolin-like
Calculated molecular weight 39 kDa
Observed molecular weight 43 kDa
GenBank accession numberNM_001747
Gene symbol CAPG
Gene ID (NCBI) 822
RRIDAB_10638618
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

Protocols

Product Specific Protocols
WB protocol for CAPG-Specific antibody 19535-1-APDownload protocol
IHC protocol for CAPG-Specific antibody 19535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Tohoku J Exp Med

Overexpression of gelsolin-like actin-capping protein is associated with progression of lung adenocarcinoma.

Authors - Shao Fangchun F
  • KD Validated
View Article