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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CAPN1 Monoclonal antibody (67732-1-Ig)

    WB analysis of HeLa using 67732-1-Ig (same clone as 67732-1-PBS)

    WB result of CAPN1 antibody (67732-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPN1 transfected HeLa cells. This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CAPN1 Monoclonal antibody (67732-1-Ig)

    WB analysis using 67732-1-Ig (same clone as 67732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67732-1-Ig (CAPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CAPN1 Monoclonal antibody (67732-1-Ig)

    WB analysis using 67732-1-Ig (same clone as 67732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67732-1-Ig (CAPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using CAPN1 Monoclonal antibody (67732-1-Ig)

    IHC staining of human urothelial carcinoma using 67732-1-Ig (same clone as 67732-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using CAPN1 Monoclonal antibody (67732-1-Ig)

    IHC staining of human urothelial carcinoma using 67732-1-Ig (same clone as 67732-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CAPN1 Monoclonal antibody (67732-1-Ig)

    IHC staining of human heart using 67732-1-Ig (same clone as 67732-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CAPN1 Monoclonal antibody (67732-1-Ig)

    IHC staining of human heart using 67732-1-Ig (same clone as 67732-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67732-1-Ig (CAPN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67732-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CAPN1 Monoclonal antibody, PBS Only

CAPN1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67732-1-PBS
Clone No.4E6H1

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67732-1-PBS targets CAPN1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CAPN1 fusion protein Ag30522 相同性解析による交差性が予測される生物種
Full Name calpain 1, (mu/I) large subunit
Calculated molecular weight 704 aa, 82 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC008751
Gene Symbol CAPN1
Gene ID (NCBI) 823
RRIDAB_2918501
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07384
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.