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Calpain 2 Polyclonal antibody

Calpain 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11472-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

CAPN2, Calpain M-type, Calpain M type, Calpain large polypeptide L2, Calpain 2 large subunit

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells, MDA-MB-231 cells, human placenta tissue, HeLa cells, Y79 cells, C2C12 cells, mouse brain tissue, rat brain tissue, K-562 cells, PC-3 cells
Positive IP detected inMDA-MB-231 cells
Positive IHC detected inhuman kidney tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11472-1-AP targets Calpain 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calpain 2 fusion protein Ag2029 相同性解析による交差性が予測される生物種
Full Name calpain 2, (m/II) large subunit
Calculated molecular weight 700 aa, 80 kDa
Observed molecular weight 72-80 kDa
GenBank accession numberBC021303
Gene Symbol CAPN2
Gene ID (NCBI) 824
RRIDAB_10755306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17655
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.

Protocols

Product Specific Protocols
WB protocol for Calpain 2 antibody 11472-1-APDownload protocol
IHC protocol for Calpain 2 antibody 11472-1-APDownload protocol
IF protocol for Calpain 2 antibody 11472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Phytomedicine

Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells

Authors - Maolei Xu
WB,IHC,IF

J Transl Med

CAPN2 promotes apalutamide resistance in metastatic hormone-sensitive prostate cancer by activating protective autophagy

Authors - Zihao Qi
mouseWB

Pharmacol Res

Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.

Authors - Juan Wang
mouseIF

Pharmacol Res

TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.

Authors - Wen Tian
mouseWB

J Am Heart Assoc

Cardiac Myocyte-Specific Overexpression of FASTKD1 Prevents Ventricular Rupture After Myocardial Infarction

Authors - Kurt D Marshall
ratWB

Front Pharmacol

Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca2+ Signaling Pathway.

Authors - Lei Liu
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