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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CAPN2 Monoclonal antibody (66977-1-Ig)

    WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CAPN2 Monoclonal antibody (66977-1-Ig)

    WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CAPN2 Monoclonal antibody (66977-1-Ig)

    WB analysis of HSC-T6 using 66977-1-Ig (same clone as 66977-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using CAPN2 Monoclonal antibody (66977-1-Ig)

    WB analysis of NIH/3T3 using 66977-1-Ig (same clone as 66977-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CAPN2 Monoclonal antibody (66977-1-Ig)

    WB analysis of human placenta using 66977-1-Ig (same clone as 66977-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CAPN2 Monoclonal antibody (66977-1-Ig)

    IHC staining of human pancreas cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CAPN2 Monoclonal antibody (66977-1-Ig)

    IHC staining of human colon cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CAPN2 Monoclonal antibody (66977-1-Ig)

    IHC staining of human stomach cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using CAPN2 Monoclonal antibody (66977-1-Ig)

    IHC staining of rat colon using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CAPN2 Monoclonal antibody (66977-1-Ig)

    IF Staining of HepG2 using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CAPN2 antibody (66977-1-Ig, Clone: 1E1F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

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CAPN2 Monoclonal antibody, PBS Only

CAPN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66977-1-PBS
Clone No.1E1F10

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Calpain 2 catalytic subunit, Calpain 2 large subunit, Calpain large polypeptide L2, Calpain M type, CANP 2, CANP2, CANPL2, CANPml, CAPN2, FLJ39928, M calpain, mCANP, Millimolar calpain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66977-1-PBS targets CAPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPN2 fusion protein Ag28427 相同性解析による交差性が予測される生物種
Full Name calpain 2, (m/II) large subunit
Calculated molecular weight 700 aa, 80 kDa
Observed molecular weight 72-80 kDa
GenBank accession numberBC021303
Gene Symbol CAPN2
Gene ID (NCBI) 824
RRIDAB_2882297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17655
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.