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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CAPN3 Monoclonal antibody, PBS Only

CAPN3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G12A5

Cat no : 67366-1-PBS

Synonyms

Calpain p94, Calpain L3, calpain 3, (p94), Calpain 3, Calcium-activated neutral proteinase 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CAPN3 Monoclonal antibody (67366-1-Ig)

    WB analysis using 67366-1-Ig (same clone as 67366-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67366-1-Ig (CAPN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CAPN3 Monoclonal antibody (67366-1-Ig)

    IHC staining of mouse skeletal muscle using 67366-1-Ig (same clone as 67366-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67366-1-Ig (CAPN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CAPN3 Monoclonal antibody (67366-1-Ig)

    IHC staining of mouse skeletal muscle using 67366-1-Ig (same clone as 67366-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67366-1-Ig (CAPN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CAPN3 Monoclonal antibody (67366-1-Ig)

    IF Staining of U2OS using 67366-1-Ig (same clone as 67366-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CAPN3 antibody (67366-1-Ig, Clone: 1G12A5 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67366-1-PBS targets CAPN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPN3 fusion protein Ag13179 相同性解析による交差性が予測される生物種
Full Name calpain 3, (p94)
Calculated molecular weight 18 kDa
Observed molecular weight 90-105 kDa, 55-60 kDa, 30-35 kDa
GenBank accession numberBC004883
Gene symbol CAPN3
Gene ID (NCBI) 825
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).