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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CAPN5 Polyclonal antibody (29809-1-AP)

    WB analysis using 29809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29809-1-AP (CAPN5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CAPN5 Polyclonal antibody (29809-1-AP)

    WB analysis using 29809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29809-1-AP (CAPN5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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CAPN5 Polyclonal antibody

CAPN5 Polyclonal Antibody for WB, ELISA
Cat No. 29809-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Synonyms

calpain 5, Calpain htra 3, CAPN5, FLJ46245, HTRA3, nCL 3, NCL3, New calpain 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, A431 cells, mouse colon tissue, A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, SH-SY5Y cells, SKOV-3 cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29809-1-AP targets CAPN5 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAPN5 fusion protein Ag30479 相同性解析による交差性が予測される生物種
Full Name calpain 5
Calculated molecular weight 640 aa, 73 kDa
Observed molecular weight65-73 kDa
GenBank accession numberBC018123
Gene Symbol CAPN5
Gene ID (NCBI) 726
RRIDAB_3086169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15484
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calpain-5 is a protein that in humans is encoded by the CAPN5 gene. Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called HTRA3.

Protocols

Product Specific Protocols
WB protocol for CAPN5 antibody 29809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols