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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calpain 7 Polyclonal antibody

Calpain 7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13870-1-AP

Synonyms

CAPN7, PalBH, EC:3.4.22.-, Calpain-7, CALPAIN7

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using Calpain 7 Polyclonal antibody (13870-1-AP)

    WB analysis of human heart using 13870-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13870-1-AP (Calpain 7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Calpain 7 Polyclonal antibody (13870-1-AP)

    IHC staining of human ovary cancer using 13870-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13870-1-AP (Calpain 7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Calpain 7 Polyclonal antibody (13870-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 13870-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13870-1-AP (Calpain 7 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13870-1-AP targets Calpain 7 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calpain 7 fusion protein Ag4820 相同性解析による交差性が予測される生物種
Full Name calpain 7
Calculated molecular weight 93 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC056202
Gene symbol Calpain 7
Gene ID (NCBI) 23473
RRIDAB_2069823
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Calpain 7 antibody 13870-1-APDownload protocol
IHC protocol for Calpain 7 antibody 13870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols