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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calpain 9 Polyclonal antibody

Calpain 9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17556-1-AP

Synonyms

NCL4, GC36, EC:3.4.22.-, Digestive tract-specific calpain, CAPN9

Validation Data Gallery

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  • Western Blot (WB) analysis of human stomach tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    WB analysis of human stomach using 17556-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    WB analysis of human stomach using 17556-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    WB analysis of mouse small intestine using 17556-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    WB analysis of human stomach using 17556-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using Calpain 9 Polyclonal antibody (17556-1-AP)

    WB analysis of HL-60 using 17556-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human stomach cancer using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human stomach cancer using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human stomach using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human stomach using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human kidney using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human kidney using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human testis using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human testis using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human lung using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human lung using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human ovary using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using Calpain 9 Polyclonal antibody (17556-1-AP)

    IHC staining of human ovary using 17556-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Calpain 9 Polyclonal antibody (17556-1-AP)

    IF Staining of HepG2 using 17556-1-AP

    Immunofluorescent analysis of HepG2 cells, using CAPN9 antibody 17556-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inhuman stomach tissue, HL-60 cells, mouse small intestine tissue
Positive IHC detected inhuman stomach cancer tissue, human kidney tissue, human lung tissue, human ovary tissue, human stomach tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17556-1-AP targets Calpain 9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calpain 9 fusion protein Ag11718 相同性解析による交差性が予測される生物種
Full Name calpain 9
Calculated molecular weight 627 aa, 72 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC027993
Gene symbol Calpain 9
Gene ID (NCBI) 10753
RRIDAB_2290833
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calpain 9 is also named as NCL4 and belongs to the peptidase C2 family.It can be down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types.It is predominantly expressed in the gastrointestinal tract and restricted to the gastric surface mucus (pit) cells in the stomach and the function of it is implicated in suppressing tumorigenesis(PMID:20686710).

Protocols

Product Specific Protocols
WB protocol for Calpain 9 antibody 17556-1-APDownload protocol
IHC protocol for Calpain 9 antibody 17556-1-APDownload protocol
IF protocol for Calpain 9 antibody 17556-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse,ratWB,IF

Front Pharmacol

Inhibition of calpain9 attenuates peritoneal dialysis-related peritoneal fibrosis

Authors - Fang Li
View Article
humanWB,IHC

Sci Rep

Decreased expression of Calpain-9 predicts unfavorable prognosis in patients with gastric cancer.

Authors - Peike Peng
View Article