Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, mouse spleen tissue |
| Positive IHC detected in | human kidney tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
Product Information
25057-1-AP targets CAPNS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CAPNS1 fusion protein Ag21885 相同性解析による交差性が予測される生物種 |
| Full Name | calpain, small subunit 1 |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC011903 |
| Gene Symbol | CAPNS1 |
| Gene ID (NCBI) | 826 |
| RRID | AB_2879876 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04632 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CAPNS1 antibody 25057-1-AP | Download protocol |
| IHC protocol for CAPNS1 antibody 25057-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate | ||
J Biol Chem Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.
| ||
Oncol Rep Capn4 promotes epithelial-mesenchymal transition in human melanoma cells through activation of the Wnt/β-catenin pathway. | ||
Cell Rep Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments. | ||
Int J Clin Exp Pathol High expression of Capn4 is associated with metastasis and poor prognosis in esophageal squamous cell carcinoma
| ||
Biomed Pharmacother Cereblon mediates macrophage differentiation and microglial phagocytosis by regulating calpain protease activity |