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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CAPRIN1 Monoclonal antibody (66352-1-Ig)

    WB analysis using 66352-1-Ig (same clone as 66352-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CAPRIN1 Monoclonal antibody (66352-1-Ig)

    IHC staining of human breast cancer using 66352-1-Ig (same clone as 66352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAPRIN1 Monoclonal antibody (66352-1-Ig)

    IHC staining of mouse brain using 66352-1-Ig (same clone as 66352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAPRIN1 Monoclonal antibody (66352-1-Ig)

    IHC staining of mouse brain using 66352-1-Ig (same clone as 66352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CAPRIN1 Monoclonal antibody (66352-1-Ig)

    IHC staining of human breast cancer using 66352-1-Ig (same clone as 66352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CAPRIN1 Monoclonal antibody (66352-1-Ig)

    IHC staining of human prostate cancer using 66352-1-Ig (same clone as 66352-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CAPRIN1 Monoclonal antibody (66352-1-Ig)

    IF Staining of HeLa using 66352-1-Ig (same clone as 66352-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (66352-1-Ig, Clone: 6B10F11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CAPRIN1 Monoclonal antibody (66352-1-Ig)

    IF Staining of HeLa using 66352-1-Ig (same clone as 66352-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66352-1-Ig(CAPRIN1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CAPRIN1 Monoclonal antibody (66352-1-Ig)

    FC experiment of NIH/3T3 using 66352-1-Ig (same clone as 66352-1-PBS)

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (66352-1-Ig, Clone:6B10F11) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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CAPRIN1 Monoclonal antibody, PBS Only

CAPRIN1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66352-1-PBS
Clone No.6B10F11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1, caprin 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66352-1-PBS targets CAPRIN1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPRIN1 fusion protein Ag7413 相同性解析による交差性が予測される生物種
Full Name cell cycle associated protein 1
Calculated molecular weight 78 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC001731
Gene Symbol CAPRIN1
Gene ID (NCBI) 4076
RRIDAB_2881732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14444
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.