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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

CAPRIN2 Polyclonal antibody

CAPRIN2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20766-1-AP

Synonyms

Gastric cancer multidrug resistance-associated protein, EEG1, Cytoplasmic activation/proliferation-associated protein 2, Caprin-2, C1QDC1

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using CAPRIN2 Polyclonal antibody (20766-1-AP)

    WB analysis of Y79 using 20766-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 20766-1-AP (CAPRIN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CAPRIN2 Polyclonal antibody (20766-1-AP)

    WB analysis of HEK-293 using 20766-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20766-1-AP (CAPRIN2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of SH-SY5Y cells using CAPRIN2 Polyclonal antibody (20766-1-AP)

    WB analysis of SH-SY5Y using 20766-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20766-1-AP (CAPRIN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using CAPRIN2 Polyclonal antibody (20766-1-AP)

    IP experiment of SH-SY5Y using 20766-1-AP

    IP result of anti-CAPRIN2 (IP:20766-1-AP, 4ug; Detection:20766-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CAPRIN2 Polyclonal antibody (20766-1-AP)

    IF Staining of HepG2 using 20766-1-AP

    Immunofluorescent analysis of HepG2 cells using 20766-1-AP (CAPRIN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CAPRIN2 Polyclonal antibody (20766-1-AP)

    IF Staining of HepG2 using 20766-1-AP

    Immunofluorescent analysis of HepG2 cells, using CAPRIN2 antibody 20766-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inY79 cells, SH-SY5Y cells, HEK-293 cells
Positive IP detected inSH-SY5Y cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20766-1-AP targets CAPRIN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAPRIN2 fusion protein Ag14745 相同性解析による交差性が予測される生物種
Full Name caprin family member 2
Calculated molecular weight 1127 aa, 126 kDa
Observed molecular weight 126-150 kDa
GenBank accession numberBC007520
Gene symbol CAPRIN2
Gene ID (NCBI) 65981
RRIDAB_10695763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CAPRIN2 antibody 20766-1-APDownload protocol
IF protocol for CAPRIN2 antibody 20766-1-APDownload protocol
IP protocol for CAPRIN2 antibody 20766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Neuroendocrinology

Transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression by CREB3L1 and CAPRIN2.

Authors - Soledad Bárez-López
View Article
mouseWB, IF

Dev Dyn

Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.

Authors - Soma Dash
  • KO Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Paul (Verified Customer) (04-29-2019)

Antibody works very well for WB and IP from HEK293T cells, and works well for WB and IF from rat tissue samples. However, I couldn't get the antibody to work for IP from rat neuronal samples.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T cells, Rat neuronal samples
CAPRIN2 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in HEK293T cells, Rat neuronal samples (Cat no:20766-1-AP)
FH

Criseyda (Verified Customer) (09-21-2018)

Two possibilities: Antibody is not detecting Carping protein well or Caprin2 is not expressed in these cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: MRC-9 Lung Fiberblasts
CAPRIN2 Antibody Western Blot, validation (1:500 dilution) in MRC-9 Lung Fiberblasts (Cat no:20766-1-AP)