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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using CAPSL Polyclonal antibody (17174-1-AP)

    WB analysis of mouse lung using 17174-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17174-1-AP (CAPSL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CAPSL Polyclonal antibody (17174-1-AP)

    WB analysis of mouse lung using 17174-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17174-1-AP (CAPSL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using CAPSL Polyclonal antibody (17174-1-AP)

    IP experiment of mouse lung using 17174-1-AP

    IP result of anti-CAPSL (IP:17174-1-AP, 4ug; Detection:17174-1-AP 1:500) with mouse lung tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CAPSL Polyclonal antibody (17174-1-AP)

    IHC staining of human lung cancer using 17174-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17174-1-AP (CAPSL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CAPSL Polyclonal antibody (17174-1-AP)

    IHC staining of human lung cancer using 17174-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17174-1-AP (CAPSL antibody) at dilution of 1:100 (under 40x lens).

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CAPSL Polyclonal antibody

CAPSL Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17174-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

Calcyphosin like protein, calcyphosine like, CAPSL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17174-1-AP targets CAPSL in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAPSL fusion protein Ag10771 相同性解析による交差性が予測される生物種
Full Name calcyphosine-like
Calculated molecular weight 198 aa, 23 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC017586
Gene Symbol CAPSL
Gene ID (NCBI) 133690
RRIDAB_2070031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWF8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CAPSL antibody 17174-1-APDownload protocol
IHC protocol for CAPSL antibody 17174-1-APDownload protocol
IP protocol for CAPSL antibody 17174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Rep

Calcyphosine-like (CAPSL) is regulated in Multiple Symmetric Lipomatosis and is involved in Adipogenesis.

Authors - Angie Lindner
View Article