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KD/KO Validated

CAPZA1 Polyclonal antibody

Name: CAPZA1 Polyclonal antibody
Brand: Proteintech
SKU: 11806-1-AP

CAPZA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11806-1-AP

Synonyms

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1

Validation Data Gallery

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AllWBIPIHC

WB analysis of human brain using 11806-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11806-1-AP (CAPZA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of SH-SY5Y using 11806-1-AP
IP result of anti-CAPZA1 (IP:11806-1-AP, 4ug; Detection:11806-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.
IHC staining of human ovary tumor using 11806-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11806-1-AP (CAPZA1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 11806-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11806-1-AP (CAPZA1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas cancer using 11806-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11806-1-AP (CAPZA1 antibody) at dilution of 1:50 (under 10x lens).

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Cat No. 11806-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human brain tissue
Positive IP detected in	SH-SY5Y cells
Positive IHC detected in	human ovary tumor tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 5 publications below
IP	See 1 publications below

Product Information

11806-1-AP targets CAPZA1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CAPZA1 fusion protein Ag2267 相同性解析による交差性が予測される生物種
Full Name	capping protein (actin filament) muscle Z-line, alpha 1
Calculated molecular weight	286 aa, 33 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC000144
Gene symbol	CAPZA1
Gene ID (NCBI)	829
RRID	AB_2070037
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)

Protocols

Product Specific Protocols
WB protocol for CAPZA1 antibody 11806-1-AP	Download protocol
IHC protocol for CAPZA1 antibody 11806-1-AP	Download protocol
IP protocol for CAPZA1 antibody 11806-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IP	J Cell Biol Heterodimeric capping protein is required for stereocilia length and width regulation. Authors - Matthew R Avenarius View Article
human	WB,IHC	Cell Mol Life Sci HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers. Authors - Gemoll Timo T View Article
human	WB	J Exp Clin Cancer Res CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma. Authors - Deng Huang KD Validated View Article
human	IHC	Invest Ophthalmol Vis Sci Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not. Authors - Linge Annett A View Article
human	WB,IHC	Int J Oncol Prognostic value of CAPZA1 overexpression in gastric cancer. Authors - Lee Young-Joon YJ KD Validated View Article
human	WB,IHC	Melanoma Res Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma. Authors - Sun Daxin D View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CAPZA1 Polyclonal antibody

CAPZA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of human brain using 11806-1-AP

IP experiment of SH-SY5Y using 11806-1-AP

IHC staining of human ovary tumor using 11806-1-AP

IHC staining of human ovary tumor using 11806-1-AP

IHC staining of human pancreas cancer using 11806-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Heterodimeric capping protein is required for stereocilia length and width regulation.

HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.

CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma.

Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not.

Prognostic value of CAPZA1 overexpression in gastric cancer.

Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma.