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  • Featured Product
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CAPZA1 Monoclonal antibody

CAPZA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

10H1F9

Cat no : 66066-1-Ig

Synonyms

capzα1, F-actin-capping protein subunit alpha-1, CapZ alpha-1, CapZ alpha 1, CAPPA1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CAPZA1 Monoclonal antibody (66066-1-Ig)

    WB analysis of HEK-293 using 66066-1-Ig

    WB result of CAPZA1 antibody (66066-1-Ig, 1:8000) with si-Control and si-CAPZA1 transfected HEK293 cells.

  • Western Blot (WB) analysis of various lysates using CAPZA1 Monoclonal antibody (66066-1-Ig)

    WB analysis using 66066-1-Ig

    Western blot analysis of CAPZA1 in various cell lines using Proteintech antibody 66066-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CAPZA1 Monoclonal antibody (66066-1-Ig)

    WB analysis of Jurkat using 66066-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66066-1-Ig (CAPZA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CAPZA1 Monoclonal antibody (66066-1-Ig)

    IHC staining of human skin cancer using 66066-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CAPZA1 Monoclonal antibody (66066-1-Ig)

    IF Staining of HeLa using 66066-1-Ig

    Immunofluorescent analysis of Hela cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CAPZA1 Monoclonal antibody (66066-1-Ig)

    IF Staining of HeLa using 66066-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CAPZA1 antibody (66066-1-Ig, Clone: 10H1F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CAPZA1 Monoclonal antibody (66066-1-Ig)

    IF Staining of MCF-7 using 66066-1-Ig

    Immunofluorescent analysis of () fixed MCF-7 cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, ROS1728 cells, PC-12 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

66066-1-Ig targets CAPZA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPZA1 fusion protein Ag18841 相同性解析による交差性が予測される生物種
Full Name capping protein (actin filament) muscle Z-line, alpha 1
Calculated molecular weight 286 aa, 33 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC000144
Gene symbol CAPZA1
Gene ID (NCBI) 829
RRIDAB_11042588
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)

Protocols

Product Specific Protocols
WB protocol for CAPZA1 antibody 66066-1-IgDownload protocol
IHC protocol for CAPZA1 antibody 66066-1-IgDownload protocol
IF protocol for CAPZA1 antibody 66066-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cancers (Basel)

Critical Role for Cold Shock Protein YB-1 in Cytokinesis.

Authors - Sunali Mehta
View Article