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KD/KO Validated

CAPZA2 Polyclonal antibody

Name: CAPZA2 Polyclonal antibody
Brand: Proteintech
SKU: 15948-1-AP

CAPZA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15948-1-AP

Synonyms

CAPPA2, CAPZ, CapZ alpha 2, CAPZA2

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of mouse brain using 15948-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15948-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15948-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15948-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 15948-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 15948-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15948-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 15948-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 15948-1-AP
IP result of anti-CAPZA2 (IP:15948-1-AP, 4ug; Detection:15948-1-AP 1:800) with mouse heart tissue lysate 4000ug.
IHC staining of human colon cancer using 15948-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 15948-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 10x lens).

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Cat No. 15948-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, HepG2 cells, MCF-7 cells, mouse heart tissue, mouse liver tissue, mouse ovary tissue, mouse skeletal muscle tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below

Product Information

15948-1-AP targets CAPZA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CAPZA2 fusion protein Ag8544 相同性解析による交差性が予測される生物種
Full Name	capping protein (actin filament) muscle Z-line, alpha 2
Calculated molecular weight	286 aa, 33 kDa
Observed molecular weight	33-40 kDa
GenBank accession number	BC005338
Gene symbol	CAPZA2
Gene ID (NCBI)	830
RRID	AB_2070148
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CAPZA2 antibody 15948-1-AP	Download protocol
IHC protocol for CAPZA2 antibody 15948-1-AP	Download protocol
IP protocol for CAPZA2 antibody 15948-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Elife HIPK4 is essential for murine spermiogenesis. Authors - J Aaron Crapster View Article
mouse	WB	Oncotarget Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer. Authors - Mi Jeong Kwon KD Validated View Article
human	WB	Biochem J Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation. Authors - João D Santos View Article
mouse	WB	Eur J Cell Biol Nuclear receptor Nur77 regulates immunomechanics of macrophages Authors - Sanne C Lith View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CAPZA2 Polyclonal antibody

CAPZA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of mouse brain using 15948-1-AP

WB analysis of mouse heart using 15948-1-AP

WB analysis of HepG2 using 15948-1-AP

WB analysis of MCF-7 using 15948-1-AP

WB analysis of mouse skeletal muscle using 15948-1-AP

WB analysis of mouse skeletal muscle using 15948-1-AP

WB analysis of mouse liver using 15948-1-AP

WB analysis of mouse ovary using 15948-1-AP

IP experiment of mouse heart using 15948-1-AP

IHC staining of human colon cancer using 15948-1-AP

IHC staining of human colon cancer using 15948-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

HIPK4 is essential for murine spermiogenesis.

Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer.

Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation.

Nuclear receptor Nur77 regulates immunomechanics of macrophages