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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CARD11 Polyclonal antibody

CARD11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28483-1-AP

Synonyms

BIMP3, CARD11, Carma 1, CARMA1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CARD11 Polyclonal antibody (28483-1-AP)

    WB analysis using 28483-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28483-1-AP (CARD11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CARD11 Polyclonal antibody (28483-1-AP)

    IHC staining of human colon cancer using 28483-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28483-1-AP (CARD11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CARD11 Polyclonal antibody (28483-1-AP)

    IHC staining of human colon cancer using 28483-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28483-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CARD11 Polyclonal antibody (28483-1-AP)

    IHC staining of human spleen using 28483-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28483-1-AP (CARD11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CARD11 Polyclonal antibody (28483-1-AP)

    IHC staining of human spleen using 28483-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28483-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, Raji cells
Positive IHC detected inhuman colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28483-1-AP targets CARD11 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARD11 fusion protein Ag29355 相同性解析による交差性が予測される生物種
Full Name caspase recruitment domain family, member 11
Calculated molecular weight 1154 aa, 133 kDa
Observed molecular weight 140-145 kDa
GenBank accession numberBC111719
Gene symbol CARD11
Gene ID (NCBI) 84433
RRIDAB_2881153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

Protocols

Product Specific Protocols
WB protocol for CARD11 antibody 28483-1-APDownload protocol
IHC protocol for CARD11 antibody 28483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Pathol Clin Res

Somatic mutations in four novel genes contribute to homologous recombination deficiency in breast cancer: a real-world clinical tumor sequencing study

Authors - Yongsheng Huang
  • KD Validated
View Article