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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CARD11 Monoclonal antibody (68269-1-Ig)

    WB analysis using 68269-1-Ig (same clone as 68269-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68269-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CARD11 Monoclonal antibody (68269-1-Ig)

    WB analysis of Raji using 68269-1-Ig (same clone as 68269-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68269-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using CARD11 Monoclonal antibody (68269-1-Ig)

    IF Staining of L02 using 68269-1-Ig (same clone as 68269-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using CARD11 antibody (68269-1-Ig, Clone: 3F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68269-1-PBS in a different storage buffer formulation.

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CARD11 Monoclonal antibody, PBS Only

CARD11 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68269-1-PBS
Clone No.3F5H6

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BIMP3, CARD11, Carma 1, CARMA1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68269-1-PBS targets CARD11 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CARD11 fusion protein Ag16468 相同性解析による交差性が予測される生物種
Full Name caspase recruitment domain family, member 11
Calculated molecular weight 1154 aa, 133 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC111719
Gene Symbol CARD11
Gene ID (NCBI) 84433
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BXL7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.