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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CARD14 Polyclonal antibody

CARD14 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28373-1-AP

Synonyms

BIMP2, CARD14, Carma 2, CARMA2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CARD14 Polyclonal antibody (28373-1-AP)

    WB analysis using 28373-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28373-1-AP (CARD14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. Daudi cells were used as a negative control.

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Tested Applications

Positive WB detected inEC109 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28373-1-AP targets CARD14 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARD14 fusion protein Ag28969 相同性解析による交差性が予測される生物種
Full Name caspase recruitment domain family, member 14
Calculated molecular weight 113 kDa
Observed molecular weight110-140 kDa
GenBank accession numberBC018142
Gene symbol CARD14
Gene ID (NCBI) 79092
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CARD14, also known as CARMA2, is closely related to CARD9, CARD10 , and CARD11 , which upon activation, each form an oligomeric CBM signaling complex with B‐cell lymphoma 10 (BCL10) and mucosa‐associated lymphoid tissue lymphoma translocation protein 1 (MALT1). It is predominantly expressed in epithelial cells of esophagus, skin, and mucosal tissues, although some isoforms were also shown to be expressed in hematopoietic cell lines.(PMID: 36009554, PMID: 32343482)

Protocols

Product Specific Protocols
WB protocol for CARD14 antibody 28373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols