Validation Data Gallery
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Tested Applications
| Positive WB detected in | EC109 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28373-1-AP targets CARD14 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CARD14 fusion protein Ag28969 相同性解析による交差性が予測される生物種 |
| Full Name | caspase recruitment domain family, member 14 |
| Calculated molecular weight | 113 kDa |
| Observed molecular weight | 110-140 kDa |
| GenBank accession number | BC018142 |
| Gene Symbol | CARD14 |
| Gene ID (NCBI) | 79092 |
| RRID | AB_3669656 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXL6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CARD14, also known as CARMA2, is closely related to CARD9, CARD10 , and CARD11 , which upon activation, each form an oligomeric CBM signaling complex with B‐cell lymphoma 10 (BCL10) and mucosa‐associated lymphoid tissue lymphoma translocation protein 1 (MALT1). It is predominantly expressed in epithelial cells of esophagus, skin, and mucosal tissues, although some isoforms were also shown to be expressed in hematopoietic cell lines.(PMID: 36009554, PMID: 32343482)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CARD14 antibody 28373-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |