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Target

Host

Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CARS Polyclonal antibody

CARS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15296-1-AP

Synonyms

Cysteinyl-tRNA synthetase, cysteinyl tRNA synthetase, Cysteine--tRNA ligase, cytoplasmic, Cysteine tRNA ligase, CYSRS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CARS Polyclonal antibody (15296-1-AP)

    WB analysis using 15296-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15296-1-AP (CARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CARS Polyclonal antibody (15296-1-AP)

    IHC staining of mouse brain using 15296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CARS Polyclonal antibody (15296-1-AP)

    IHC staining of mouse brain using 15296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CARS Polyclonal antibody (15296-1-AP)

    IHC staining of human pancreas cancer using 15296-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CARS Polyclonal antibody (15296-1-AP)

    IHC staining of human pancreas cancer using 15296-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CARS Polyclonal antibody (15296-1-AP)

    IF Staining of MCF-7 using 15296-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CARS antibody 15296-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inmouse brain tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15296-1-AP targets CARS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARS fusion protein Ag7546 相同性解析による交差性が予測される生物種
Full Name cysteinyl-tRNA synthetase
Calculated molecular weight 89 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC002880
Gene symbol CARS
Gene ID (NCBI) 833
RRIDAB_2068455
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CARS antibody 15296-1-APDownload protocol
IHC protocol for CARS antibody 15296-1-APDownload protocol
IF protocol for CARS antibody 15296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

CARS senses cysteine deprivation to activate AMPK for cell survival.

Authors - Mengqiu Yuan
  • KD Validated
View Article
humanWB,IP

Ann Rheum Dis

Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies

Authors - Jean-Baptiste Vulsteke
View Article