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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CARS Monoclonal antibody

CARS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F7E4

Cat no : 67933-1-Ig

Synonyms

CARS, CARS1, CYSRS, Cysteine tRNA ligase, cysteinyl tRNA synthetase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CARS Monoclonal antibody (67933-1-Ig)

    WB analysis using 67933-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using CARS Monoclonal antibody (67933-1-Ig)

    WB analysis using 67933-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of A549 cells using CARS Monoclonal antibody (67933-1-Ig)

    WB analysis of A549 using 67933-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CARS Monoclonal antibody (67933-1-Ig)

    IF Staining of HepG2 using 67933-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CARS antibody (67933-1-Ig, Clone: 2F7E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA431 cells, A549 cells, HSC-T6 cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67933-1-Ig targets CARS in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CARS fusion protein Ag7651 相同性解析による交差性が予測される生物種
Full Name cysteinyl-tRNA synthetase
Calculated molecular weight 89 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC002880
Gene symbol CARS
Gene ID (NCBI) 833
RRIDAB_2918685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CARS antibody 67933-1-IgDownload protocol
IF protocol for CARS antibody 67933-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols