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CASK Polyclonal antibody

CASK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55277-1-AP

Synonyms

CAGH39, CASK, CMG, FGS4, hCASK, LIN2, MICPCH, Protein lin 2 homolog, TNRC8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CASK Polyclonal antibody (55277-1-AP)

    WB analysis using 55277-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CASK Polyclonal antibody (55277-1-AP)

    WB analysis of A549 using 55277-1-AP

    WB result of CASK antibody (55277-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CASK transfected A549 cells.

  • Western Blot (WB) analysis of mouse liver tissue using CASK Polyclonal antibody (55277-1-AP)

    WB analysis of mouse liver using 55277-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CASK Polyclonal antibody (55277-1-AP)

    WB analysis of mouse brain using 55277-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CASK Polyclonal antibody (55277-1-AP)

    IHC staining of human thyroid cancer using 55277-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using CASK Polyclonal antibody (55277-1-AP)

    IHC staining of rat small intestine using 55277-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CASK Polyclonal antibody (55277-1-AP)

    IHC staining of mouse kidney using 55277-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse liver tissue, A549 cells, rat brain tissue
Positive IHC detected inrat small intestine tissue, mouse kidney tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55277-1-AP targets CASK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent serine protein kinase (MAGUK family)
Calculated molecular weight 105 kDa
Observed molecular weight 100-110 kDa, 50-55 kDa
GenBank accession numberNM_003688
Gene symbol CASK
Gene ID (NCBI) 8573
RRIDAB_10858487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CASK, also named as LIN2, belongs to the MAGUK family. It is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. It contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. CASK binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. It may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) . Defects in CASK are the cause of FG syndrome type 4 (FGS4). This antibody can recognize all the isoform of CASK.

Protocols

Product Specific Protocols
WB protocol for CASK antibody 55277-1-APDownload protocol
IHC protocol for CASK antibody 55277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols