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Caspase 1/p20/p10 Polyclonal antibody

Caspase 1/p20/p10 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22915-1-AP

Synonyms

CASP 1, CASP1, Caspase 1, Caspase 1/p20/p10, Caspase1, ICE, IL 1 beta converting enzyme, IL 1BC, IL1BC, IL1BCE, p10, p20, P45



Tested Applications

Positive WB detected inRaji cells, HeLa cells, C6 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, HepG2 cells, THP-1 cells, PC-12 cells, U-937 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

22915-1-AP targets Caspase 1/p20/p10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse, monkey, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 1/p20/p10 fusion protein Ag11143 相同性解析による交差性が予測される生物種
Full Name caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
Calculated molecular weight 404 aa, 45 kDa
Observed molecular weight 45-47 kDa, 30 kDa, 35 kDa
GenBank accession numberBC062327
Gene symbol CASP1
Gene ID (NCBI) 834
RRIDAB_2876874
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize all the five isoforms of caspase 1. It also can recognize p45 procaspase and the mature fragments p10 and p20.

Protocols

Product Specific Protocols
WB protocol for Caspase 1/p20/p10 antibody 22915-1-APDownload protocol
IHC protocol for Caspase 1/p20/p10 antibody 22915-1-APDownload protocol
IF protocol for Caspase 1/p20/p10 antibody 22915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cancer

Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases

Authors - Qiang Wang
humanWB

ACS Nano

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Authors - Zhiwei Liao
mouseWB,IF

Mol Cell

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Authors - Chuanlong Wang
mouseWB

Nat Commun

REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis.

Authors - Shuai Wang
mouseWB

Theranostics

Ablation of NLRP3 inflammasome attenuates muscle atrophy via inhibiting pyroptosis, proteolysis and apoptosis following denervation

Authors - Zongqi You
mouseWB

J Clin Invest

Melatonin inhibits cytosolic mitochondrial DNA-induced neuroinflammatory signaling in accelerated aging and neurodegeneration.

Authors - Abhishek Jauhari

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joseph (Verified Customer) (02-19-2020)

This antibody detected pro-caspase 1 and the p20 cleaved product in PDAC cell lines. I did not look for the p10 cleaved product.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: PDAC cell lines
FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain