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Caspase 1/p20/p10 Polyclonal antibody

Caspase 1/p20/p10 Polyclonal Antibody for WB, IHC, ELISA, IF
Cat No. 22915-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (6)

Applications

WB, IHC, IF, IP, ELISA

CASP1, Caspase1, Caspase-1, Caspase 1, CASP-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inRaji cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, THP-1 cells, PC-12 cells, U-937 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22915-1-AP targets Caspase 1/p20/p10 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig, canine, monkey, chicken, bovine, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 1/p20/p10 fusion protein Ag11143 相同性解析による交差性が予測される生物種
Full Name caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
Calculated molecular weight 404 aa, 45 kDa
Observed molecular weight 45-47 kDa, 30 kDa, 35 kDa
GenBank accession numberBC062327
Gene Symbol Caspase 1
Gene ID (NCBI) 834
RRIDAB_2876874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29466
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize all the five isoforms of caspase 1. It also can recognize p45 procaspase and the mature fragments p10 and p20.

Protocols

Product Specific Protocols
WB protocol for Caspase 1/p20/p10 antibody 22915-1-APDownload protocol
IHC protocol for Caspase 1/p20/p10 antibody 22915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cancer

Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases

Authors - Qiang Wang
humanWB

ACS Nano

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Authors - Zhiwei Liao
mouseWB,IF

Mol Cell

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Authors - Chuanlong Wang
humanWB

J Hazard Mater

Adverse outcome pathway-based approach to reveal the mechanisms of skin sensitization and long-term aging effects of chlorothalonil

Authors - Yung-Hsuan Cheng
IHC

J Hazard Mater

Perinatal exposure to PBEB aggravates liver injury via macrophage-derived TWEAK in male adult offspring mice under western diet

Authors - Wanyue Wang
mouseWB

Nat Commun

REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis.

Authors - Shuai Wang
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