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- KD/KO Validated
Caspase 4/p20/p10 Polyclonal antibody
Caspase 4/p20/p10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 11856-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | SGC-7901 cells, THP-1 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 12 publications below |
| IHC | See 2 publications below |
Product Information
11856-1-AP targets Caspase 4/p20/p10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Caspase 4/p20/p10 fusion protein Ag2429 相同性解析による交差性が予測される生物種 |
| Full Name | caspase 4, apoptosis-related cysteine peptidase |
| Calculated molecular weight | 377 aa, 43 kDa |
| Observed molecular weight | 43-48 kDa |
| GenBank accession number | BC017839 |
| Gene symbol | Caspase 4 |
| Gene ID (NCBI) | 837 |
| RRID | AB_2070053 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 4/p20/p10 antibody 11856-1-AP | Download protocol |
| IHC protocol for Caspase 4/p20/p10 antibody 11856-1-AP | Download protocol |
| IF protocol for Caspase 4/p20/p10 antibody 11856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma
| ||
Front Microbiol Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4. | ||
Front Microbiol Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells. | ||
Oral Dis Palmitic acid induces GSDMD-mediated pyroptosis in periodontal ligament cells via the NF-κB pathway | ||
Chem Res Toxicol Atypical Gasdermin D and Mixed Lineage Kinase Domain-like Protein Leakage Aggravates Tetrachlorobenzoquinone-Induced Nod-like Receptor Protein 3 Inflammasome Activation. | ||
