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KD/KO Validated

Caspase 4/p20/p10 Polyclonal antibody

Name: Caspase 4/p20/p10 Polyclonal antibody
Brand: Proteintech
SKU: 11856-1-AP

Caspase 4/p20/p10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11856-1-AP

Synonyms

CASP4, Caspase4, Caspase-4, Caspase 4, CASP-4

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of SGC-7901 using 11856-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11856-1-AP (CASP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 11856-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11856-1-AP (Caspase 4/p20/p10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 11856-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11856-1-AP (Caspase 4/p20/p10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 11856-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11856-1-AP (CASP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 11856-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11856-1-AP (CASP4 antibody) at dilution of 1:50 (under 25x lens).
IF Staining of HepG2 using 11856-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 4/p20/p10 antibody (11856-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Cat No. 11856-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SGC-7901 cells, THP-1 cells
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 13 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

11856-1-AP targets Caspase 4/p20/p10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Caspase 4/p20/p10 fusion protein Ag2429 相同性解析による交差性が予測される生物種
Full Name	caspase 4, apoptosis-related cysteine peptidase
Calculated molecular weight	377 aa, 43 kDa
Observed molecular weight	43-48 kDa
GenBank accession number	BC017839
Gene symbol	Caspase 4
Gene ID (NCBI)	837
RRID	AB_2070053
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

Protocols

Product Specific Protocols
WB protocol for Caspase 4/p20/p10 antibody 11856-1-AP	Download protocol
IHC protocol for Caspase 4/p20/p10 antibody 11856-1-AP	Download protocol
IF protocol for Caspase 4/p20/p10 antibody 11856-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC,WB	Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma Authors - Liangmin Fu KD Validated View Article
human	WB	Front Microbiol Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4. Authors - Pei-Hui Ding View Article
human	WB	Front Microbiol Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells. Authors - Bei Wang View Article
human	WB,IF	Arch Biochem Biophys GSDMB involvement in the pathogenesis of abdominal aortic aneurysm through regulation of macrophage non-canonical pyroptosis Authors - Xiaoping Xie View Article
human	WB	Oral Dis Palmitic acid induces GSDMD-mediated pyroptosis in periodontal ligament cells via the NF-κB pathway Authors - Bing-Jing Sun View Article
human	WB	Chem Res Toxicol Atypical Gasdermin D and Mixed Lineage Kinase Domain-like Protein Leakage Aggravates Tetrachlorobenzoquinone-Induced Nod-like Receptor Protein 3 Inflammasome Activation. Authors - Xiaomin Xia View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Caspase 4/p20/p10 Polyclonal antibody

Caspase 4/p20/p10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of SGC-7901 using 11856-1-AP

WB analysis using 11856-1-AP

WB analysis using 11856-1-AP

WB analysis of SGC-7901 using 11856-1-AP

IHC staining of human pancreas cancer using 11856-1-AP

IF Staining of HepG2 using 11856-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma

Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.

Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.

GSDMB involvement in the pathogenesis of abdominal aortic aneurysm through regulation of macrophage non-canonical pyroptosis

Palmitic acid induces GSDMD-mediated pyroptosis in periodontal ligament cells via the NF-κB pathway

Atypical Gasdermin D and Mixed Lineage Kinase Domain-like Protein Leakage Aggravates Tetrachlorobenzoquinone-Induced Nod-like Receptor Protein 3 Inflammasome Activation.