Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of Jurkat using 13423-1-AP
Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 13423-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13423-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 13423-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of UV treated Hela using 13423-1-AP
UV treated Hela cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 13423-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 13423-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13423-1-AP
IP result of anti-Caspase 8/p43/p18 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug.
IP experiment of Jurkat using 13423-1-AP
IP result of anti-Caspase 8/p43/p18 (IP:13423-1-AP, 4ug; Detection:13423-1-AP 1:500) with Jurkat cells lysate 2880 ug.
IHC staining of human lymphoma using 13423-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 13423-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 13423-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 13423-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lymphoma using 13423-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 13423-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 13423-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 13423-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 8/p43/p18 antibody (13423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

Caspase 8/p43/p18 Polyclonal antibody

Caspase 8/p43/p18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13423-1-AP

Publications(345)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

CASP8, Caspase 8, CASP-8, CAP4, Apoptotic protease Mch-5

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (345)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Staurosporine treated Jurkat cells, RAW264.7, HeLa cells, Raji cells, Jurkat cells, UV treated Hela, Sp2/0 cells
Positive IP detected in	HeLa cells, Jurkat cells
Positive IHC detected in	human lymphoma tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 327 publications below
IHC	See 20 publications below
IF	See 11 publications below
IP	See 3 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

13423-1-AP targets Caspase 8/p43/p18 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Caspase 8/p43/p18 fusion protein Ag4214 相同性解析による交差性が予測される生物種
Full Name	caspase 8, apoptosis-related cysteine peptidase
Calculated molecular weight	538 aa, 62 kDa
Observed molecular weight	53-57 kDa, 32-45 kDa
GenBank accession number	BC028223
Gene Symbol	Caspase 8
Gene ID (NCBI)	841
RRID	AB_2068463
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14790
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.

Protocols

Product Specific Protocols
WB protocol for Caspase 8/p43/p18 antibody 13423-1-AP	Download protocol
IHC protocol for Caspase 8/p43/p18 antibody 13423-1-AP	Download protocol
IF protocol for Caspase 8/p43/p18 antibody 13423-1-AP	Download protocol
IP protocol for Caspase 8/p43/p18 antibody 13423-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model Authors - Mengyan Hu View Article
	IP	Sci Adv HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling. Authors - Chun Gao View Article
human	WB	Exp Mol Med PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer Authors - Gu-Choul Shin View Article
human	WB	Nat Commun Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms. Authors - Christoph Grohmann View Article
human	WB	Aging Dis MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis. Authors - Yanhui Cui View Article
human	WB	EMBO J A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination. Authors - Xu Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 13423-1-AP

WB analysis using 13423-1-AP

WB analysis of HeLa using 13423-1-AP

WB analysis of RAW264.7 using 13423-1-AP

WB analysis of UV treated Hela using 13423-1-AP

WB analysis of Raji using 13423-1-AP

WB analysis of RAW264.7 using 13423-1-AP

IP experiment of HeLa using 13423-1-AP

IP experiment of Jurkat using 13423-1-AP

IHC staining of human lymphoma using 13423-1-AP

IHC staining of human lymphoma using 13423-1-AP

IHC staining of human lymphoma using 13423-1-AP

IHC staining of human lymphoma using 13423-1-AP

IHC staining of human lymphoma using 13423-1-AP

IHC staining of human colon using 13423-1-AP

IHC staining of human colon using 13423-1-AP

IF Staining of HepG2 using 13423-1-AP

Caspase 8/p43/p18 Polyclonal antibody

Caspase 8/p43/p18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.

PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer

Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms.

MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis.

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.