Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse testis tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| ChIP | See 1 publications below |
Product Information
11336-1-AP targets CBFA2T2 in WB, IHC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CBFA2T2 fusion protein Ag1880 相同性解析による交差性が予測される生物種 |
| Full Name | core-binding factor, runt domain, alpha subunit 2; translocated to, 2 |
| Calculated molecular weight | 604 aa, 67 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC015066 |
| Gene Symbol | CBFA2T2 |
| Gene ID (NCBI) | 9139 |
| RRID | AB_2070571 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43439 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Core-binding factor, runt domain, alpha subunit 2, translocated to 2(CBFA2T2) belongs to a family of ubiquitously expressed transcriptional repressors that interact with transcription factors bound to promoters of target genes and with histone deacetylases (HDACs) critical for enforcement of repressor function.CBFA2T2, also named as MTGR1 or EHT, is an 85-kDa phosphoprotein. It can bind AML1-MTG8 fusion protein in acute myeloid leukemia. The binding leads to an enhancement of cell proliferation mediated in a murine myeloid model. The calculated molecular weight of CBFA2T2 is 67 kDa, but modified SMARCE1 is about 80 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CBFA2T2 antibody 11336-1-AP | Download protocol |
| IHC protocol for CBFA2T2 antibody 11336-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|