Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 25818-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis using 25818-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 25818-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 25818-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 25818-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 25818-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 25818-1-AP
IP result of anti-c-Cbl (IP:25818-1-AP, 4ug; Detection:25818-1-AP 1:1000) with K-562 cells lysate 3680 ug.
IP experiment of K-562 using 25818-1-AP
IP result of anti-c-Cbl (IP:25818-1-AP, 4ug; Detection:25818-1-AP 1:1000) with K-562 cells lysate 3680 ug.
IHC staining of human tonsillitis using 25818-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 25818-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 25818-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 25818-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 25818-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

c-Cbl Polyclonal antibody

c-Cbl Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 25818-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

CBL, EC:2.3.2.27, E3 ubiquitin-protein ligase CBL, CBL2, Casitas B-lineage lymphoma proto-oncogene

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, Raji cells, Ramos cells, Romas cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human tonsillitis tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 18 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 3 publications below
CoIP	See 4 publications below

Product Information

25818-1-AP targets c-Cbl in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	c-Cbl fusion protein Ag23033 相同性解析による交差性が予測される生物種
Full Name	Cas-Br-M (murine) ecotropic retroviral transforming sequence
Calculated molecular weight	906 aa, 100 kDa
Observed molecular weight	100-120 kDa
GenBank accession number	BC132733
Gene Symbol	CBL
Gene ID (NCBI)	867
RRID	AB_2880252
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22681
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

Protocols

Product Specific Protocols
WB protocol for c-Cbl antibody 25818-1-AP	Download protocol
IHC protocol for c-Cbl antibody 25818-1-AP	Download protocol
IP protocol for c-Cbl antibody 25818-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Hepatology PMID: 29729186 Authors - Huixia Zhang KD Validated View Article
human	WB,IF,CoIP	Cell Rep Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis Authors - Huan Li KD Validated View Article
human	WB	Cell Death Dis ZC3H15 promotes glioblastoma progression through regulating EGFR stability. Authors - Jianbing Hou View Article
human	WB	Cell Commun Signal Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells Authors - Limei Xu View Article
mouse	WB	Front Med Targeting deubiquitinase OTUB1 protects vascular smooth muscle cells in atherosclerosis by modulating PDGFRβ Authors - Fei Xu View Article
human	WB	Oncogene AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression Authors - Sicheng Wan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 25818-1-AP

WB analysis using 25818-1-AP

WB analysis of K-562 using 25818-1-AP

WB analysis of K-562 using 25818-1-AP

WB analysis of Raji using 25818-1-AP

WB analysis of K-562 using 25818-1-AP

IP experiment of K-562 using 25818-1-AP

IP experiment of K-562 using 25818-1-AP

IHC staining of human tonsillitis using 25818-1-AP

IHC staining of human lymphoma using 25818-1-AP

IHC staining of human lymphoma using 25818-1-AP

IHC staining of human tonsillitis using 25818-1-AP

IHC staining of human tonsillitis using 25818-1-AP

c-Cbl Polyclonal antibody

c-Cbl Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PMID: 29729186

Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis

ZC3H15 promotes glioblastoma progression through regulating EGFR stability.

Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells

Targeting deubiquitinase OTUB1 protects vascular smooth muscle cells in atherosclerosis by modulating PDGFRβ

AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression