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KD/KO Validated

CBLB Polyclonal antibody

Name: CBLB Polyclonal antibody
Brand: Proteintech
SKU: 12781-1-AP

CBLB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12781-1-AP

Synonyms

RING-type E3 ubiquitin transferase CBL-B, RING finger protein 56, Nbla00127, EC:2.3.2.27, E3 ubiquitin-protein ligase CBL-B

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 12781-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12781-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12781-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12781-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12781-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12781-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12781-1-AP (CBLB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 12781-1-AP
IP result of anti-CBLB (IP:12781-1-AP, 4ug; Detection:12781-1-AP 1:600) with HEK-293 cells lysate 1800 ug.
IHC staining of mouse colon using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB antibody) at dilution of 1:50 (under 10x lens)..
IHC staining of mouse colon using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12781-1-AP (CBLB antibody) at dilution of 1:50 (under 40x lens)..
IHC staining of human kidney using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 12781-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 12781-1-AP (CBLB antibody) at dilution of 1:100 (under 40x lens).
IF Staining of A431 using 12781-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBLB antibody (12781-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 12781-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, A549 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	mouse colon tissue, human kidney tissue, human placenta tissue, human testis tissue, human spleen tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 13 publications below
IHC	See 2 publications below
IF	See 1 publications below
IP	See 3 publications below
FC	See 1 publications below
CoIP	See 1 publications below

Product Information

12781-1-AP targets CBLB in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CBLB fusion protein Ag3491 相同性解析による交差性が予測される生物種
Full Name	Cas-Br-M (murine) ecotropic retroviral transforming sequence b
Calculated molecular weight	982 aa, 109 kDa
Observed molecular weight	110-130 kDa
GenBank accession number	BC032851
Gene symbol	CBLB
Gene ID (NCBI)	868
RRID	AB_2275326
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CBLB(E3 ubiquitin-protein ligase CBL-B) is also named RNF56 and plays a necessary role in preventing the inappropriate activation of NF-B in response to lipopolysaccharide and polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and various types of cell stimulation. It also has a crucial role in preventing chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing 109, 90, and 86 kDa, and observed molecular weight at 110-130 kDa.

Protocols

Product Specific Protocols
WB protocol for CBLB antibody 12781-1-AP	Download protocol
IHC protocol for CBLB antibody 12781-1-AP	Download protocol
IF protocol for CBLB antibody 12781-1-AP	Download protocol
IP protocol for CBLB antibody 12781-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Exp Med Cbl-b deficiency prevents functional but not phenotypic T cell anergy. Authors - Trang T T Nguyen View Article
human	WB	Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. Authors - Hongwei Chu View Article
rat	IHC	Sci Signal Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn. Authors - Zi-Yang Zhang View Article
human	FC	J Immunol A Multiple Sclerosis-Associated Variant of CBLB Links Genetic Risk with Type I IFN Function. Authors - Klarissa Hanja Stürner View Article
mouse	WB,IF	J Cell Mol Med NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection. Authors - Ze-Qing Pu View Article
mouse	WB	Biomolecules miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles. Authors - Xin Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBLB Polyclonal antibody

CBLB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 12781-1-AP

WB analysis of A549 using 12781-1-AP

WB analysis of A549 using 12781-1-AP

WB analysis of HEK-293 using 12781-1-AP

WB analysis of HEK-293 using 12781-1-AP

WB analysis of A549 using 12781-1-AP

IP experiment of HEK-293 using 12781-1-AP

IHC staining of mouse colon using 12781-1-AP

IHC staining of mouse colon using 12781-1-AP

IHC staining of human kidney using 12781-1-AP

IHC staining of human kidney using 12781-1-AP

IHC staining of human placenta using 12781-1-AP

IHC staining of human placenta using 12781-1-AP

IHC staining of human testis using 12781-1-AP

IHC staining of human testis using 12781-1-AP

IHC staining of human spleen using 12781-1-AP

IHC staining of human spleen using 12781-1-AP

IHC staining of human lung using 12781-1-AP

IHC staining of human lung using 12781-1-AP

IF Staining of A431 using 12781-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cbl-b deficiency prevents functional but not phenotypic T cell anergy.

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.

A Multiple Sclerosis-Associated Variant of CBLB Links Genetic Risk with Type I IFN Function.

NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.

miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles.