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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBLL1 Polyclonal antibody

CBLL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29219-1-AP

Synonyms

c Cbl like protein 1, CBLL1, FLJ23109, HAKAI, RING finger protein 188, RNF188

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBLL1 Polyclonal antibody (29219-1-AP)

    WB analysis using 29219-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29219-1-AP (CBLL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CBLL1 Polyclonal antibody (29219-1-AP)

    IHC staining of human lung cancer using 29219-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29219-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, NCI-H1299 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29219-1-AP targets CBLL1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBLL1 fusion protein Ag30900 相同性解析による交差性が予測される生物種
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1
Calculated molecular weight 491 aa, 55 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC027460
Gene symbol CBLL1
Gene ID (NCBI) 79872
RRIDAB_2918251
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Cbl proto‐oncogene E3 ubiquitin protein ligase‐like 1, RNF188, Hakai (CBLL1) is an evolutionarily conserved E3 ubiquitin ligase containing a RING‐finger domain. CBLL1 contains a typical RING‐finger, short pTyr‐binding domain, and proline‐rich domain. CBLL1 expression is upregulated in human colon and gastric cancer tissues, and CBLL1 has been reported to induce anchorage‐dependent cell growth. CBLL1 immunostaining was observed in both the nuclei and cytoplasm of cancer cells. (PMID: 31124298, PMID: 31646569, PMID: 21191016)

Protocols

Product Specific Protocols
WB protocol for CBLL1 antibody 29219-1-APDownload protocol
IHC protocol for CBLL1 antibody 29219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols