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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using CBLN1 Polyclonal antibody (18207-1-AP)

    WB analysis of SH-SY5Y using 18207-1-AP

    WB result of CBLN1 antibody (18207-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBLN1 transfected SH-SY5Y cells.

  • Western Blot (WB) analysis of various lysates using CBLN1 Polyclonal antibody (18207-1-AP)

    WB analysis using 18207-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18207-1-AP (CBLN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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CBLN1 Polyclonal antibody

CBLN1 Polyclonal Antibody for WB, ELISA
Cat No. 18207-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CBLN1, CER [des Ser1] cerebellin, Cerebellin 1, cerebellin 1 precursor, Precerebellin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, U-87 MG cells, Neuro-2a cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18207-1-AP targets CBLN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBLN1 fusion protein Ag12835 相同性解析による交差性が予測される生物種
Full Name cerebellin 1 precursor
Calculated molecular weight 193 aa, 21 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC093692
Gene Symbol CBLN1
Gene ID (NCBI) 869
RRIDAB_3669294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23435
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CBLN1 (Cerebellin-1) is also named as Precerebellin. CBLN1 is a newly identified synaptic organizer belonging to the C1q family. Cbln1 was recently identified as the missing ligand for the orphan glutamate receptor δ2 (GluD2) (PMID: 21342763). In the cerebellum, Cbln1 is produced and secreted from granule cells and works as a strong synapse organizer between Purkinje cells and parallel fibers, the axons of the granule cells (PMID: 24607546). The glycosylation modification of CBLN1 resulted in a larger molecular weight.

Protocols

Product Specific Protocols
WB protocol for CBLN1 antibody 18207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols