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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBLN4 Polyclonal antibody

CBLN4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20559-1-AP

Synonyms

CBLN4, CBLNL1, Cerebellin 4, cerebellin 4 precursor, Cerebellin like glycoprotein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using CBLN4 Polyclonal antibody (20559-1-AP)

    WB analysis of mouse cerebellum using 20559-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 20559-1-AP (CBLN4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CBLN4 Polyclonal antibody (20559-1-AP)

    WB analysis of mouse brain using 20559-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20559-1-AP (CBLN4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using CBLN4 Polyclonal antibody (20559-1-AP)

    WB analysis of mouse kidney using 20559-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20559-1-AP (CBLN4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using CBLN4 Polyclonal antibody (20559-1-AP)

    WB analysis of mouse kidney using 20559-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20559-1-AP (CBLN4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using CBLN4 Polyclonal antibody (20559-1-AP)

    IHC staining of human cerebellum using 20559-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using CBLN4 Polyclonal antibody (20559-1-AP)

    IHC staining of human cerebellum using 20559-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CBLN4 Polyclonal antibody (20559-1-AP)

    IHC staining of human testis using 20559-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CBLN4 Polyclonal antibody (20559-1-AP)

    IHC staining of human testis using 20559-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse cerebellum tissue, mouse brain tissue, mouse kidney tissue
Positive IHC detected inhuman cerebellum tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20559-1-AP targets CBLN4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cerebellin 4 precursor
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberNM_080617
Gene symbol CBLN4
Gene ID (NCBI) 140689
RRIDAB_2878701
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CBLN4 may be involved in synaptic functions in the CNS. CBLN4 can enable ER export and secretion of CBLN3. The antibody has no cross reaction to CBLN1, 2, or 3.

Protocols

Product Specific Protocols
WB protocol for CBLN4 antibody 20559-1-APDownload protocol
IHC protocol for CBLN4 antibody 20559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols