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KD/KO Validated

NCBP1 Polyclonal antibody

Name: NCBP1 Polyclonal antibody
Brand: Proteintech
SKU: 10349-1-AP

NCBP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10349-1-AP

Synonyms

CBP80, NCBP, NCBP 80 kDa subunit, NCBP1

Validation Data Gallery

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AllWBIPIHC

WB analysis using 10349-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10349-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10349-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10349-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10349-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10349-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10349-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10349-1-AP
IP result of anti-NCBP1 (IP:10349-1-AP, 4ug; Detection:10349-1-AP 1:500) with HeLa cells lysate 2480ug.
IHC staining of human lung cancer using 10349-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10349-1-AP (NCBP1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 10349-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10349-1-AP (NCBP1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 10349-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10349-1-AP (NCBP1 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 10349-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, human brain tissue, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 12 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 3 publications below

Product Information

10349-1-AP targets NCBP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NCBP1 fusion protein Ag0496 相同性解析による交差性が予測される生物種
Full Name	nuclear cap binding protein subunit 1, 80kDa
Calculated molecular weight	92 kDa
Observed molecular weight	80 kDa
GenBank accession number	BC001450
Gene symbol	NCBP1
Gene ID (NCBI)	4686
RRID	AB_2150127
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.

Protocols

Product Specific Protocols
WB protocol for NCBP1 antibody 10349-1-AP	Download protocol
IHC protocol for NCBP1 antibody 10349-1-AP	Download protocol
IP protocol for NCBP1 antibody 10349-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock. Authors - Fustin Jean-Michel JM KD Validated View Article
mouse	WB,IP	Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits Authors - Yuanjun Li View Article
human	WB	Mol Cell Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay. Authors - Hanae Sato View Article
human	WB,IP	Genes Dev SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay. Authors - Yamashita Akio A View Article
human	WB	Nucleic Acids Res SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function. Authors - Yuzhang Du View Article
human	WB	Sci Signal AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay. Authors - Izumi Natsuko N View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NCBP1 Polyclonal antibody

NCBP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 10349-1-AP

WB analysis of HeLa using 10349-1-AP

WB analysis of HEK-293 using 10349-1-AP

WB analysis of human brain using 10349-1-AP

WB analysis of HeLa using 10349-1-AP

WB analysis of HEK-293 using 10349-1-AP

WB analysis of HeLa using 10349-1-AP

IP experiment of HeLa using 10349-1-AP

IHC staining of human lung cancer using 10349-1-AP

IHC staining of human kidney using 10349-1-AP

IHC staining of human kidney using 10349-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock.

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay.

SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.

SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.

AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay.