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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBR1 Recombinant antibody (83736-4-RR)

    WB analysis using 83736-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83736-4-RR (CBR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBR1 Recombinant antibody (83736-4-RR)

    IF Staining of HeLa using 83736-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CBR1 antibody (83736-4-RR, Clone: 240707G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83736-4-RR

    Affinity and Kinetic Characterization of 83736-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83736-4-RR against Human CBR1 were performed. The affinity constant is 13.7 nM.

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CBR1 Recombinant antibody

CBR1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83736-4-RR
Clone No.240707G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:1.1.1.184, carbonyl reductase 1, Alcohol dehydrogenase [NAD(P)+] CBR1, 240707G1, 20-beta-hydroxysteroid dehydrogenase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293T cells, MCF-7 cells, SKOV-3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83736-4-RR targets CBR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CBR1 fusion protein Ag34830 相同性解析による交差性が予測される生物種
Full Name carbonyl reductase 1
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC002511
Gene Symbol CBR1
Gene ID (NCBI) 873
RRIDAB_3671336
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16152
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CBR1 belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics.

Protocols

Product Specific Protocols
WB protocol for CBR1 antibody 83736-4-RRDownload protocol
IF protocol for CBR1 antibody 83736-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols