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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBR3 Polyclonal antibody, PBS Only

CBR3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15619-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBR3 Polyclonal antibody (15619-1-AP)

    WB analysis using 15619-1-AP (same clone as 15619-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15619-1-AP (CBR3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CBR3 Polyclonal antibody (15619-1-AP)

    WB analysis of Jurkat using 15619-1-AP (same clone as 15619-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15619-1-AP (CBR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CBR3 Polyclonal antibody (15619-1-AP)

    IHC staining of human colon cancer using 15619-1-AP (same clone as 15619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15619-1-AP (CBR3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CBR3 Polyclonal antibody (15619-1-AP)

    IHC staining of human colon cancer using 15619-1-AP (same clone as 15619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15619-1-AP (CBR3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBR3 Polyclonal antibody (15619-1-AP)

    IF Staining of HeLa using 15619-1-AP (same clone as 15619-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15619-1-AP (CBR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15619-1-PBS targets CBR3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBR3 fusion protein Ag8039 相同性解析による交差性が予測される生物種
Full Name carbonyl reductase 3
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC002812
Gene symbol CBR3
Gene ID (NCBI) 874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CBR3(Carbonyl reductase [NADPH] 3), which Belongs to the short-chain dehydrogenases/reductases (SDR) family, has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). The gene encodes a 31 kDa protein that can catalyze the reduction of many endogenous and xenobiotic carbonyl compounds, including steroids and prostaglandins. The deduced 277-amino acid CBR3 protein is 84% similar to the CBR1 protein(PMID:9740676).