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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBR4 Polyclonal antibody

CBR4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13725-1-AP

Synonyms

carbonyl reductase 4, CBR4, Quinone reductase CBR4, SDR45C1

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using CBR4 Polyclonal antibody (13725-1-AP)

    WB analysis of BxPC-3 using 13725-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 13725-1-AP (CBR4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CBR4 Polyclonal antibody (13725-1-AP)

    WB analysis of HepG2 using 13725-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13725-1-AP (CBR4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CBR4 Polyclonal antibody (13725-1-AP)

    IP experiment of HepG2 using 13725-1-AP

    IP result of anti-CBR4 (IP:13725-1-AP, 4ug; Detection:13725-1-AP 1:300) with HepG2 cells lysate 3400ug.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using CBR4 Polyclonal antibody (13725-1-AP)

    IHC staining of human hepatocirrhosis using 13725-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13725-1-AP (CBR4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using CBR4 Polyclonal antibody (13725-1-AP)

    IHC staining of human hepatocirrhosis using 13725-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13725-1-AP (CBR4 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inBxPC-3 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13725-1-AP targets CBR4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBR4 fusion protein Ag4678 相同性解析による交差性が予測される生物種
Full Name carbonyl reductase 4
Calculated molecular weight 237 aa, 25 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC033650
Gene symbol CBR4
Gene ID (NCBI) 84869
RRIDAB_10643240
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CBR4 antibody 13725-1-APDownload protocol
IHC protocol for CBR4 antibody 13725-1-APDownload protocol
IP protocol for CBR4 antibody 13725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols