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  • Featured Product
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CBS Monoclonal antibody

CBS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E4F10

Cat no : 67861-1-Ig

Synonyms

Beta thionase, CBS, cystathionine beta synthase, HIP4, Serine sulfhydrase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBS Monoclonal antibody (67861-1-Ig)

    WB analysis using 67861-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67861-1-Ig (CBS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using CBS Monoclonal antibody (67861-1-Ig)

    WB analysis of NCI-H1299 using 67861-1-Ig

    WB result of CBS antibody (67861-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBS transfected NCI-H1299 cells.

  • Western Blot (WB) analysis of HEK-293 cells using CBS Monoclonal antibody (67861-1-Ig)

    WB analysis of HEK-293 using 67861-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67861-1-Ig (CBS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CBS Monoclonal antibody (67861-1-Ig)

    WB analysis of Jurkat using 67861-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67861-1-Ig (CBS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CBS Monoclonal antibody (67861-1-Ig)

    WB analysis of K-562 using 67861-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67861-1-Ig (CBS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CBS Monoclonal antibody (67861-1-Ig)

    IHC staining of human pancreas cancer using 67861-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67861-1-Ig (CBS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CBS Monoclonal antibody (67861-1-Ig)

    IHC staining of human pancreas cancer using 67861-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67861-1-Ig (CBS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBS Monoclonal antibody (67861-1-Ig)

    IF Staining of HeLa using 67861-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBS antibody (67861-1-Ig, Clone: 1E4F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBS Monoclonal antibody (67861-1-Ig)

    IF Staining of HeLa using 67861-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CBS antibody (67861-1-Ig, Clone: 1E4F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CBS Monoclonal antibody (67861-1-Ig)

    IF Staining of HeLa using 67861-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CBS antibody (67861-1-Ig, Clone: 1E4F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, NCI-H1299 cells, HeLa cells, THP-1 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67861-1-Ig targets CBS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityrat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CBS fusion protein Ag7008 相同性解析による交差性が予測される生物種
Full Name cystathionine-beta-synthase
Calculated molecular weight 61 kDa
Observed molecular weight 61-63 kDa
GenBank accession numberBC000440
Gene symbol CBS
Gene ID (NCBI) 875
RRIDAB_2918619
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).

Protocols

Product Specific Protocols
WB protocol for CBS antibody 67861-1-IgDownload protocol
IHC protocol for CBS antibody 67861-1-IgDownload protocol
IF protocol for CBS antibody 67861-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

FEBS J

Treatment with aripiprazole and N-acetylcysteine affects anaerobic cysteine metabolism in the hippocampus and reverses schizophrenia-like behavior in the neurodevelopmental rat model of schizophrenia

Authors - Magdalena Górny
View Article
ratWB

Biomed Pharmacother

Hydrogen sulfide ameliorated preeclampsia via suppression of toll-like receptor 4-activated inflammation in the rostral ventrolateral medulla of rats.

Authors - Jiexian Du
View Article