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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBX1 Polyclonal antibody (31108-1-AP)

    WB analysis using 31108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31108-1-AP (CBX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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CBX1 Polyclonal antibody

CBX1 Polyclonal Antibody for WB, ELISA
Cat No. 31108-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

HP1Hsbeta, HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31108-1-AP targets CBX1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBX1 fusion protein Ag34901 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 1 (HP1 beta homolog Drosophila )
Calculated molecular weight 27 kDa
Observed molecular weight21-27 kDa
GenBank accession numberBC002609
Gene Symbol CBX1
Gene ID (NCBI) 10951
RRIDAB_3669859
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP83916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CBX1 (chromobox 1), also known as CBX. It is expected to be located in nucleus, the protein is expressed in all adult and embryonic tissues. It is a member of the heterochromatin protein family. CBX1 is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. It may play an important role in the epigenetic control of chromatin structure and gene expression. The calculated molecular weight of CBX1 is 21 kDa.

Protocols

Product Specific Protocols
WB protocol for CBX1 antibody 31108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols