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  • KD/KO Validated

CBX4 Polyclonal antibody

CBX4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA, PLA

Conjugate

Unconjugated

Cat no : 18544-1-AP

Synonyms

Pc2, NBP16, hPc2, EC:2.3.2.-, E3 SUMO-protein ligase CBX4


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Tested Applications

Positive WB detected inVarious cell, HEK-293 cell, PC-3 cells
Positive IF detected inU2OS cell, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18544-1-AP targets CBX4 in WB, IF, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBX4 fusion protein Ag13425 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 4 (Pc class homolog, Drosophila)
Calculated molecular weight 61 kDa
Observed molecular weight ~75 kDa
GenBank accession numberBC014967
Gene symbol CBX4
Gene ID (NCBI) 8535
RRIDAB_2878552
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.

Protocols

Product Specific Protocols
WB protocol for CBX4 antibody 18544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,PLA

Front Cell Dev Biol

SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes.

Authors - Immacolata Giordano
humanWB

Bioengineered

Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis.

Authors - Zengxi Yang
  • KD Validated
humanWB

Mol Oncol

CBX4 promotes anti-tumor immunity by suppressing Pdcd1 expression in T cells

Authors - Liwei Ren