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  • Featured Product
  • KD/KO Validated

CBX4 Polyclonal antibody, PBS Only

CBX4 Polyclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18544-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cell using CBX4 Polyclonal antibody (18544-1-AP)

    WB analysis of HEK-293 cell using 18544-1-AP (same clone as 18544-1-PBS)

    WB result of anti-CBX4 (18544-1-AP) with various cell. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE). This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using CBX4 Polyclonal antibody (18544-1-AP)

    WB analysis of PC-3 using 18544-1-AP (same clone as 18544-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CBX4 Polyclonal antibody (18544-1-AP)

    WB analysis of HEK-293 using 18544-1-AP (same clone as 18544-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using CBX4 Polyclonal antibody (18544-1-AP)

    WB analysis of PC-3 using 18544-1-AP (same clone as 18544-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18544-1-AP (CBX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cell using CBX4 Polyclonal antibody (18544-1-AP)

    IF Staining of U2OS cell using 18544-1-AP (same clone as 18544-1-PBS)

    IF result of anti-CBX4 (18544-1-AP, 1:200) with U2OS cell, MeOH fixation. (Courtesy of L. Pirone and R. Barrio, CIC bioGUNE). This data was developed using the same antibody clone with 18544-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18544-1-PBS targets CBX4 in WB, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBX4 fusion protein Ag13425 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 4 (Pc class homolog, Drosophila)
Calculated molecular weight 61 kDa
Observed molecular weight ~75 kDa
GenBank accession numberBC014967
Gene symbol CBX4
Gene ID (NCBI) 8535
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CBX4, also named as E3 SUMO-protein ligase CBX4 or Polycomb 2 homolog, is a 560 amino acid protein, which contains 1 chromo domain. CBX4 facilitates SUMO1 conjugation by UBE2I. CBX4 acting as a transcriptional co-repressor is involved in maintaining the transcriptionally repressive state of genes, modifies chromatin, rendering it heritably changed in its expressibility. CBX4 localizes in the nucleus and is widely expressed in various tissues. The calculated molecular weight of CBX4 is 61 kDa, but the modified CBX4 may be a 70-75 kDa protein.