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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBX5 Recombinant antibody, PBS Only (Detector)

CBX5 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240145F11

Cat no : 83258-5-PBS

Synonyms

HP1A, HP1 alpha, HP1, Antigen p25, 240145F11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBX5 Recombinant antibody (83258-5-RR)

    WB analysis using 83258-5-RR (same clone as 83258-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83258-5-RR (CBX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CBX5 Recombinant antibody (83258-5-RR)

    IF Staining of HepG2 using 83258-5-RR (same clone as 83258-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-5-RR, Clone: 240145F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CBX5 Recombinant antibody (83258-5-RR)

    FC experiment of SH-SY5Y using 83258-5-RR (same clone as 83258-5-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug CBX5 Recombinant antibody (83258-5-RR, Clone:240145F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83258-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00181-3

    Cytometric bead array standard curve of MP00181-3

    Cytometric bead array standard curve ofMP00181-3, CBX5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83258-2-PBS. Detection antibody: 83258-5-PBS. Standard: Ag34810. Range: 3.125-100 ng/mL.

  • Affinity and Kinetic Characterization of 83258-5-RR

    Affinity and Kinetic Characterization of 83258-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83258-5-RR against Human CBX5 were performed. The affinity constant is 0.339 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83258-5-PBS targets CBX5 as part of a matched antibody pair:

MP00181-3: 83258-2-PBS capture and 83258-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CBX5 fusion protein Ag34810 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 5 (HP1 alpha homolog, Drosophila)
Calculated molecular weight 191 aa, 22 kDa
GenBank accession numberBC006821
Gene symbol CBX5
Gene ID (NCBI) 23468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.