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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CBX5 Recombinant antibody, PBS Only

CBX5 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240145G12

Cat no : 83258-6-PBS

Synonyms

Antigen p25, CBX5, Chromobox protein homolog 5, HP1, HP1 alpha, HP1A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBX5 Recombinant antibody (83258-6-RR)

    WB analysis using 83258-6-RR (same clone as 83258-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83258-6-RR (CBX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83258-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CBX5 Recombinant antibody (83258-6-RR)

    IF Staining of HepG2 using 83258-6-RR (same clone as 83258-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-6-RR, Clone: 240145G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83258-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CBX5 Recombinant antibody (83258-6-RR)

    IF Staining of HepG2 using 83258-6-RR (same clone as 83258-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX5 antibody (83258-6-RR, Clone: 240145G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83258-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83258-6-PBS

    Affinity and Kinetic Characterization of 83258-6-PBS

    Biolayer interferometry (BLl) kinetic assays of 83258-6-RR against Human CBX5 were performed. The affinity constant is 0.606 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83258-6-PBS is CBX5 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CBX5 fusion protein Ag34810 相同性解析による交差性が予測される生物種
Full Name chromobox homolog 5 (HP1 alpha homolog, Drosophila)
Calculated molecular weight 191 aa, 22 kDa
GenBank accession numberBC006821
Gene symbol CBX5
Gene ID (NCBI) 23468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.