CBX6 Polyclonal antibody
CBX6 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 23426-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
The immunogen of 23426-1-AP is CBX6 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CBX6 fusion protein Ag20014 相同性解析による交差性が予測される生物種 |
| Full Name | chromobox homolog 6 |
| Calculated molecular weight | 412 aa, 44 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC064900 |
| Gene symbol | CBX6 |
| Gene ID (NCBI) | 23466 |
| RRID | AB_2737364 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The Chromobox domain (Cbx) gene family, consisting of Polycomb and Heterochromatin Protein 1 genes, involves in transcriptional repression, cell cycle regulation and chromatin remodeling [PMID: 17486619]. Cbx6, other named neuronal pentraxin receptor (NPR), first described as a putative integral membrane pentraxin that interacts with neuronal pentraxin 1 and 2, has also been classified as a Pc protein [PMID: 14647293]. NPR was determined to be a member of a distinct pentraxin protein subfamily, and found to form heteropentamer swithNP1 andNP2, followed by release from cell membranes [PMID: 10748068].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CBX6 antibody 23426-1-AP | Download protocol |
| IHC protocol for CBX6 antibody 23426-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors. |
