Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of mouse liver using 12239-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12239-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12239-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12239-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12239-1-AP (CBY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 12239-1-AP
IP result of anti-CBY1 (IP:12239-1-AP, 4ug; Detection:12239-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IHC staining of human kidney using 12239-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 12239-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 12239-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 12239-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 12239-1-AP (CBY1 antibody) at dilution of 1:50 (under 40x lens).

CBY1 Polyclonal antibody

CBY1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12239-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

arb1, ARPP binding protein, C22orf2, CBY, CBY1, chibby homolog 1 (Drosophila), Cytosolic leucine rich protein, HS508I15A, PGEA1, PIGEA 14, PIGEA14, Protein chibby homolog 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, mouse kidney tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human kidney tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

12239-1-AP targets CBY1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CBY1 fusion protein Ag2882 相同性解析による交差性が予測される生物種
Full Name	chibby homolog 1 (Drosophila)
Calculated molecular weight	126 aa, 14 kDa
Observed molecular weight	30-35 kDa
GenBank accession number	BC016139
Gene Symbol	CBY1
Gene ID (NCBI)	25776
RRID	AB_10951858
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y3M2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CBY1 antibody 12239-1-AP	Download protocol
IHC protocol for CBY1 antibody 12239-1-AP	Download protocol
IP protocol for CBY1 antibody 12239-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Cell Biol Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation. Authors - Michael C Burke View Article
mouse,human	WB,IF	Dev Dyn DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism. Authors - Lilong Guo View Article
human	IHC	Medicine (Baltimore) Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma Authors - Gang Ren View Article
human	IF	Adv Sci (Weinh) The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers Authors - Huicheng Chen View Article
human	WB	Heliyon MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling Authors - Qingqing Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse liver using 12239-1-AP

WB analysis of mouse kidney using 12239-1-AP

WB analysis of mouse kidney using 12239-1-AP

WB analysis of mouse liver using 12239-1-AP

IP experiment of mouse liver using 12239-1-AP

IHC staining of human kidney using 12239-1-AP

IHC staining of human kidney using 12239-1-AP

IHC staining of human heart using 12239-1-AP

IHC staining of human heart using 12239-1-AP

CBY1 Polyclonal antibody

CBY1 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.

DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism.

Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma

The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers

MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling