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KD/KO Validated

CC2D1A Polyclonal antibody

Name: CC2D1A Polyclonal antibody
Brand: Proteintech
SKU: 16816-1-AP

CC2D1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16816-1-AP

Synonyms

FREUD 1, FRE under dual repression-binding protein 1, Five prime repressor element under dual repression-binding protein 1, Coiled-coil and C2 domain-containing protein 1A, Akt kinase-interacting protein 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 16816-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16816-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16816-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16816-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16816-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 16816-1-AP
IP result of anti-CC2D1A (IP:16816-1-AP, 4ug; Detection:16816-1-AP 1:2000) with mouse liver tissue lysate 4000ug.
IHC staining of human testis using 16816-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 16816-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 16816-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 16816-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 16816-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 16816-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 16816-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 16816-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 16816-1-AP
Immunofluorescent analysis of HepG2 cells, using CC2D1A antibody 16816-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 16816-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, Jurkat cells, mouse liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human testis tissue, human brain tissue, human liver tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below

Product Information

16816-1-AP targets CC2D1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CC2D1A fusion protein Ag10355 相同性解析による交差性が予測される生物種
Full Name	coiled-coil and C2 domain containing 1A
Calculated molecular weight	951 aa, 104 kDa
Observed molecular weight	130 kDa, 104 kDa
GenBank accession number	BC064981
Gene symbol	CC2D1A
Gene ID (NCBI)	54862
RRID	AB_2275394
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CC2D1A (coiled-coil and C2 domain-containing 1A), also known as Freud-1, Aki1 or TAPE (TBK1-associated protein in endolysosomes), is a evolutionary conserved protein located in different subcellular compartments, including the nucleus, centrosome, and endolysosomes. It acts as a scaffold protein that interacts with various proteins and plays diverse biological roles. Mutations in CC2D1A have been linked to nonsyndromic mental retardation (NMSR) and generate a truncated 85-kDa product. Several isoforms of CC2D1A proteins have also been described: 120 /130-kDa doublet of long isoform and 67 kDa short isoform. The short isoform of CC2D1A has been identified as the predominant isoform in rodent cells, while the long isoform is more abundant in human cells. Recently it has been reported that CC2D1A/TAPE is the first innate immune regulator implicated in both TLR and RLR signaling at a very early step.

Protocols

Product Specific Protocols
WB protocol for CC2D1A antibody 16816-1-AP	Download protocol
IHC protocol for CC2D1A antibody 16816-1-AP	Download protocol
IF protocol for CC2D1A antibody 16816-1-AP	Download protocol
IP protocol for CC2D1A antibody 16816-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Anesthesiology Astroglial MicroRNA-219-5p in the Ventral Tegmental Area Regulates Nociception in Rats. Authors - Song Zhang View Article
mouse	WB	J Biol Chem TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity. Authors - Chen Kuan-Ru KR KD Validated View Article
mouse	WB	Neurotherapeutics Loss of CC2D1A in Glutamatergic Neurons Results in Autistic-Like Features in Mice. Authors - Cheng-Yi Yang View Article
mouse	WB	J Neurosci Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation. Authors - Cheng-Yi Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CC2D1A Polyclonal antibody

CC2D1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 16816-1-AP

WB analysis of Jurkat using 16816-1-AP

WB analysis of HeLa using 16816-1-AP

WB analysis of HeLa using 16816-1-AP

WB analysis of mouse liver using 16816-1-AP

IP experiment of mouse liver using 16816-1-AP

IHC staining of human testis using 16816-1-AP

IHC staining of human testis using 16816-1-AP

IHC staining of human skin using 16816-1-AP

IHC staining of human skin using 16816-1-AP

IHC staining of human brain using 16816-1-AP

IHC staining of human brain using 16816-1-AP

IHC staining of human liver using 16816-1-AP

IHC staining of human liver using 16816-1-AP

IF Staining of HepG2 using 16816-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Astroglial MicroRNA-219-5p in the Ventral Tegmental Area Regulates Nociception in Rats.

TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity.

Loss of CC2D1A in Glutamatergic Neurons Results in Autistic-Like Features in Mice.

Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation.