CC2D2A Polyclonal antibody
CC2D2A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 22293-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
22293-1-AP targets CC2D2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil and C2 domain containing 2A |
| Calculated molecular weight | 186 kDa |
| Observed molecular weight | 190 kDa |
| GenBank accession number | NM_020785 |
| Gene symbol | CC2D2A |
| Gene ID (NCBI) | 57545 |
| RRID | AB_2879063 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CC2D2A, also named as KIAA1345, may be involved in cilia formation. CC2D2A encodes a protein with similar overall structure to RPGRIP1L, including coiled-coil domains, a C2 domain, and an overlapping centrosomal protein-related domain. CC2D2A physically interacts with CEP290, and loss of Cc2d2a function in the zebrafish sentinel mutant results in abnormal body shape and pronephric (kidney) cysts that is strongly exacerbated by knockdown of Cep290 function. (PMID:19778711 )Defects in CC2D2A are the cause of Meckel syndrome type 6 (MKS6). Defects in CC2D2A are the cause of Joubert syndrome type 9 (JBTS9). The antibody is specific to CC2D2A.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CC2D2A antibody 22293-1-AP | Download protocol |
| IHC protocol for CC2D2A antibody 22293-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia. | ||
Ann Hum Genet Identification of pathogenic deep intronic variant and exonic LINE-1 insertion in a patient with Meckel syndrome |
