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  • Featured Product
  • KD/KO Validated

CCAR2 Monoclonal antibody, PBS Only

CCAR2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D10F8

Cat no : 66497-1-PBS

Synonyms

cell cycle and apoptosis regulator 2, DBC1, DBC-1, KIAA1967, DBC.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCAR2 Monoclonal antibody (66497-1-Ig)

    WB analysis using 66497-1-Ig (same clone as 66497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CCAR2 Monoclonal antibody (66497-1-Ig)

    WB analysis of HEK-293 using 66497-1-Ig (same clone as 66497-1-PBS)

    WB result of CCAR2 antibody (66497-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCAR2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    WB analysis of pig brain using 66497-1-Ig (same clone as 66497-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human lung cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human lung cancer using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCAR2 Monoclonal antibody (66497-1-Ig)

    IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66497-1-Ig (CCAR2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCAR2 Monoclonal antibody (66497-1-Ig)

    IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCAR2 antibody (66497-1-Ig, Clone: 2D10F8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using CCAR2 Monoclonal antibody (66497-1-Ig)

    FC experiment of U2OS using 66497-1-Ig (same clone as 66497-1-PBS)

    1X10^6 U2OS cells were intracellularly stained with 0.2 ug Anti-Human CCAR2 (66497-1-Ig, Clone:2D10F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66497-1-PBS targets CCAR2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCAR2 fusion protein Ag18374 相同性解析による交差性が予測される生物種
Full Name KIAA1967
Calculated molecular weight 923 aa, 103 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC018269
Gene symbol CCAR2
Gene ID (NCBI) 57805
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).