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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCBL1 Polyclonal antibody

CCBL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30296-1-AP

Synonyms

CCBL1, FLJ95217, Glutamine transaminase K, GTK, KAT1, KATI, Kynurenine aminotransferase I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCBL1 Polyclonal antibody (30296-1-AP)

    WB analysis using 30296-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30296-1-AP (CCBL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCBL1 Polyclonal antibody (30296-1-AP)

    IF Staining of HeLa using 30296-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CCBL1 antibody (30296-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, U-87 MG cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30296-1-AP targets CCBL1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCBL1 fusion protein Ag32316 相同性解析による交差性が予測される生物種
Full Name cysteine conjugate-beta lyase, cytoplasmic
Observed molecular weight 48 kDa
GenBank accession numberBC033685
Gene symbol CCBL1
Gene ID (NCBI) 883
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCBL1 encodes kynurenine aminotransferase 1 (KAT1), a key enzyme involved in kynurenine pathway. CCBL1 catalyzes the production of kynurenic acid, a powerful endogenous excitatory amino acid receptor antagonist that is widely regarded as the potent neuroprotective agent.

Protocols

Product Specific Protocols
WB protocol for CCBL1 antibody 30296-1-APDownload protocol
IF protocol for CCBL1 antibody 30296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Hua (Verified Customer) (08-30-2024)

Works well in WB with mouse liver lysate. Background is clean. The signal is not very strong.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse liver
CCBL1 Antibody Western Blot validation (1:500 dilution) in Mouse liver (Cat no:30296-1-AP)