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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCBL1 Polyclonal antibody (30296-1-AP)

    WB analysis using 30296-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30296-1-AP (CCBL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCBL1 Polyclonal antibody (30296-1-AP)

    IF Staining of HeLa using 30296-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CCBL1 antibody (30296-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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CCBL1 Polyclonal antibody

CCBL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30296-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

CCBL1, FLJ95217, Glutamine transaminase K, GTK, KAT1, KATI, Kynurenine aminotransferase I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, U-87 MG cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30296-1-AP targets CCBL1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCBL1 fusion protein Ag32316 相同性解析による交差性が予測される生物種
Full Name cysteine conjugate-beta lyase, cytoplasmic
Observed molecular weight 48 kDa
GenBank accession numberBC033685
Gene Symbol CCBL1
Gene ID (NCBI) 883
RRIDAB_2935538
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16773
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCBL1 encodes kynurenine aminotransferase 1 (KAT1), a key enzyme involved in kynurenine pathway. CCBL1 catalyzes the production of kynurenic acid, a powerful endogenous excitatory amino acid receptor antagonist that is widely regarded as the potent neuroprotective agent.

Protocols

Product Specific Protocols
WB protocol for CCBL1 antibody 30296-1-APDownload protocol
IF protocol for CCBL1 antibody 30296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols