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  • Featured Product
  • KD/KO Validated

SGF29 Polyclonal antibody

SGF29 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24061-1-AP

Synonyms

CCDC101, SGF29

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SGF29 Polyclonal antibody (24061-1-AP)

    WB analysis of HeLa using 24061-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24061-1-AP (SGF29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using SGF29 Polyclonal antibody (24061-1-AP)

    WB analysis of MDA-MB-453s using 24061-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24061-1-AP (SGF29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SGF29 Polyclonal antibody (24061-1-AP)

    IHC staining of human liver cancer using 24061-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SGF29 Polyclonal antibody (24061-1-AP)

    IHC staining of human liver cancer using 24061-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SGF29 Polyclonal antibody (24061-1-AP)

    IHC staining of human cervical cancer using 24061-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SGF29 Polyclonal antibody (24061-1-AP)

    IHC staining of human cervical cancer using 24061-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 40x lens).

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Tested Applications

Positive WB detected inHeLa cells, MDA-MB-453s cells
Positive IHC detected inhuman liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24061-1-AP targets SGF29 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGF29 fusion protein Ag21261 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 101
Calculated molecular weight 293 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC011981
Gene symbol SGF29
Gene ID (NCBI) 112869
RRIDAB_2879420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SAGA-associated factor 29 (SGF29) is a chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes. SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PMID:19103755, PMID:26421618, PMID:21685874, PMID:26578293). Involved in the response to the endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed:23894581). It has a calculated molecular weight of 33 kDa.

Protocols

Product Specific Protocols
WB protocol for SGF29 antibody 24061-1-APDownload protocol
IHC protocol for SGF29 antibody 24061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Authors - Lingyun Long
  • KO Validated
View Article