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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CCDC105 Polyclonal antibody

CCDC105 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 24026-1-AP

Synonyms

TEKTL1, Tektin-like protein 1, FLJ40365, Coiled-coil domain-containing protein 105

Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using CCDC105 Polyclonal antibody (24026-1-AP)

    WB analysis of DU 145 using 24026-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CCDC105 Polyclonal antibody (24026-1-AP)

    WB analysis of SH-SY5Y using 24026-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CCDC105 Polyclonal antibody (24026-1-AP)

    WB analysis of HeLa using 24026-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC105 Polyclonal antibody (24026-1-AP)

    IHC staining of human testis using 24026-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CCDC105 Polyclonal antibody (24026-1-AP)

    IHC staining of human testis using 24026-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CCDC105 Polyclonal antibody (24026-1-AP)

    IF Staining of mouse testis using 24026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CCDC105 Polyclonal antibody (24026-1-AP)

    IF Staining of mouse testis using 24026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCDC105 Polyclonal antibody (24026-1-AP)

    IF Staining of HeLa using 24026-1-AP

    Immunofluorescent analysis of HeLa cells using 24026-1-AP (CCDC105 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inDU 145 cells, HeLa cells, SH-SY5Y cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24026-1-AP targets CCDC105 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC105 fusion protein Ag21208 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 105
Calculated molecular weight 499 aa, 57 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC035678
Gene symbol CCDC105
Gene ID (NCBI) 126402
RRIDAB_2879406
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCDC105 antibody 24026-1-APDownload protocol
IHC protocol for CCDC105 antibody 24026-1-APDownload protocol
IF protocol for CCDC105 antibody 24026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols