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  • Featured Product
  • KD/KO Validated

CCDC111/PRIMPOL Polyclonal antibody, PBS Only

CCDC111/PRIMPOL Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29824-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCDC111/PRIMPOL Polyclonal antibody (29824-1-AP)

    WB analysis using 29824-1-AP (same clone as 29824-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CCDC111/PRIMPOL Polyclonal antibody (29824-1-AP)

    WB analysis of HepG2 using 29824-1-AP (same clone as 29824-1-PBS)

    WB result of CCDC111/PRIMPOL antibody (29824-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC111/PRIMPOL transfected HepG2 cells. This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CCDC111/PRIMPOL Polyclonal antibody (29824-1-AP)

    WB analysis using 29824-1-AP (same clone as 29824-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CCDC111/PRIMPOL Polyclonal antibody (29824-1-AP)

    IHC staining of human kidney using 29824-1-AP (same clone as 29824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CCDC111/PRIMPOL Polyclonal antibody (29824-1-AP)

    IF Staining of U2OS using 29824-1-AP (same clone as 29824-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCDC111/PRIMPOL antibody (29824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29824-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29824-1-PBS targets CCDC111/PRIMPOL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC111/PRIMPOL fusion protein Ag31478 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 111
Observed molecular weight64 kDa
GenBank accession numberBC064600
Gene symbol CCDC111/PRIMPOL
Gene ID (NCBI) 201973
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CCDC111, also known as PRIMPOL, FLJ33167, which contains the active-site motifs of AEP primases and a putative Zn finger similar to that of herpesvirus UL52 primase and other AEP-like enzymes. CCDC111 is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions (PMID: 24126761, PMID: 24207056). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (Uniprot, PMID: 30715459).