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Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEP89, CCDC123 Monoclonal antibody

CEP89, CCDC123 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F12C5

Cat no : 68112-1-Ig

Synonyms

CEP89, CCDC123, 1F12C5, Cep123, Centrosomal protein of 89 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using CEP89, CCDC123 Monoclonal antibody (68112-1-Ig)

    WB analysis of Neuro-2a using 68112-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CEP89, CCDC123 Monoclonal antibody (68112-1-Ig)

    WB analysis using 68112-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CEP89, CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Western Blot (WB) analysis of various lysates using CEP89, CCDC123 Monoclonal antibody (68112-1-Ig)

    WB analysis using 68112-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using CEP89, CCDC123 Monoclonal antibody (68112-1-Ig)

    IF Staining of mouse eye using 68112-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using CEP89, CCDC123 Monoclonal antibody (68112-1-Ig)

    IF Staining of mouse eye using 68112-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CEP89, CCDC123 Monoclonal antibody (68112-1-Ig)

    IF Staining of hTERT-RPE1 using 68112-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, Neuro-2a cells, A431 cells, SH-5Y5Y cells, U-251 cells, SH-SY5Y cells
Positive IF-P detected inmouse eye tissue, hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68112-1-Ig targets CEP89, CCDC123 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CEP89, CCDC123 fusion protein Ag28339 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 123
Calculated molecular weight 783 aa, 90 kDa
Observed molecular weight90 kDa
GenBank accession numberBC136328
Gene symbol CEP89
Gene ID (NCBI) 84902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC123 (as known as CEP123), also named as CEP89, encodes for a protein required for ciliogenesis. It plays a role in mitochondrial metabolism by modulating complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with CEP290 (PMID:23575228, 23789104, 23348840).

Protocols

Product Specific Protocols
WB protocol for CEP89, CCDC123 antibody 68112-1-IgDownload protocol
IF protocol for CEP89, CCDC123 antibody 68112-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols